Precautions for the use of ultrasonic cell disruptor
The ultrasonic cell disruptor converts electrical energy into sound energy through a transducer. This energy passes through the liquid medium and becomes dense small bubbles.
Precautions for the use of ultrasonic cell disruptor:
1. Avoid no-load (must insert the ultrasonic horn into the sample before turning on the machine)
2. Water penetration depth of the horn (ultrasonic probe): about 1.5cm, the height of the liquid level is above 30mm, the probe should be centered, and not attached to the wall. Ultrasound is a vertical longitudinal wave, and it is not easy to form convection if it is inserted too deep, which affects the crushing efficiency.
3. Ultrasonic parameter setting: set the working parameters of the instrument. For samples that are sensitive to temperature (such as bacteria), ice bath is generally used outside. The actual temperature must be lower than 25 degrees, and protein nucleic acid will definitely not be denatured.
3.1. Time: The ultrasonic time should not exceed 5 seconds each time, and the gap time should be greater than or equal to the ultrasonic time to facilitate heat dissipation. The time setting should be based on the principle of short ultrasound time and multiple ultrasound times, which can extend the life of the ultrasound machine and probe.
3.2. Ultrasonic power: It should not be too large to avoid splashing or foaming of the sample. If the sample capacity is less than 10ml, the power should be within 200w. Use a 2mm ultrasonic probe and turn the horn selector switch behind the panel to the corresponding block; 10- If the power of 200ml sample capacity is 200-400w, choose 6mm ultrasonic probe, and turn the horn behind the panel to the corresponding gear; if the power of 200ml or more sample capacity is between 300-600w, choose 10mm ultrasonic probe, and put it behind the panel. To the corresponding gear; (2MM small probe power is strictly prohibited to exceed 350W)
3.3. Container selection: Choose a beaker as much as there are samples, which is also beneficial for sample convection in ultrasound and improve crushing efficiency. For example, use a 20ML beaker for a 20ML processing volume.
Such as 100ml E. coli sample setting parameters: ultrasonic 5 seconds / interval of 5 seconds, 70 times (total time 10 minutes). Power 300W. About 500ML, the power is about 500W-800W.
4. If the sample is placed in a 1.5ml EP tube, be sure to fix the EP tube to prevent the liquid level from falling after the ice bath melts and causing no load.
5. Daily maintenance: scrub the probe with alcohol or ultrasound with clean water after use.