Application of micro-jet homogenizer in biological industry
In the field of biology, there are often applications of cell disruption to extract substances, such as E. coli, yeast cells and algae cells, to extract proteins and intracellular enzymes and other active substances. Commonly used cell disruption methods include ultrasonic, chemical, enzymatic and high-pressure homogenization Methods such as chemical method and enzymatic hydrolysis are non-mechanical methods due to the need to introduce other reactive substances, which makes the process more complicated, while ultrasonic method and high-speed grinding method are not suitable for large-scale production due to the crushing strength. High-pressure homogenization method uses high pressure The homogenizer generates extremely high homogenizing pressure, collides the cells in a narrow channel at super high speed, and opens the cell membrane or cell wall to dissolve the active substances that need to be extracted. It is a purely physical process without the need to add additional chemicals. , And it is easy to perfect the output to the scale of production. Especially the micro-jet homogenizer, as a new generation of high-pressure homogenizer, greatly increases the homogenization design pressure (up to 300MPA), and can cover a variety of difficult-to-break cells ( Yeast, fungal cells). Commonly used brands include American BEE, Genizer and Microfluidics, etc., and the equipment quality, operability and stability are relatively good. Here are a few examples of the application of microfluidic homogenizers in the biological industry to break cells. Described.
1. Spirulina contains a variety of beneficial nutrients to the human body and can be easily obtained through large-area mariculture. Its rich algae protein has great application potential in the cosmetics and food industries. The use of microjets to homogenize and break algae cells can be effective The production-type scale of algae protein needs to be optimized for the process of homogenization pressure, homogenization times, algae cell concentration, buffer pH value, suspension system and other factors. According to experience, the micro-jet homogenizer is used under the pressure of 20000-30000PSI Better results can be achieved by breaking 3 times.
2. Escherichia coli is a commonly used bacterium in cell disruption and is often used for protein extraction. Since the cell membrane of E. coli is relatively easy to break, the single-shot fragmentation rate can reach more than 99% under a pressure of about 15000 PSI with a micro-jet homogenizer. After high-pressure homogenization, the temperature of the material will increase, and the degree of temperature increase will become more severe with the increase of the homogenization pressure. Therefore, the back end of the homogeneous crushing needs to be connected with a heat exchange device to reduce the temperature of the material to a suitable level in time to ensure the activity of the protein At the same time, it is best to design a cooling jacket for the homogeneous point of heat.
3. Yeast cells also have high nucleic acid, which can be used in medicine, food and cosmetics industries, and the beer industry has a huge amount of waste beer yeast mud that can be used. It can not only dig deeper into the economic value of yeast, but also solve feedstuffs. Yeast has the problem of high nucleic acid. The industrial extraction methods of brewer's yeast commonly use concentrated salt method, ammonia method and sodium hydroxide method, but they can not be widely promoted due to low effect or use of a large number of chemical substances. Microjet homogenizers are used. Under the pressure of 25000-30000PSI, the wall breaking rate of about 80%-90% can be achieved by homogenizing three times.
The above are some of the cell disruption applications in the biological industry.In addition, there are many cases that can be homogenized by microjets, such as animal cell disruption and fungal cell disruption.
Nanogenizer 30K, as an experimental micro-jet homogenizer developed by Genizer, USA, has a maximum operating pressure of 200MPA and a design flow of 100ml/min. It is mainly used for research and development of universities, research institutes, and enterprises. It is equipped with a unique counter-jet diamond interactive cavity. Excellent homogenization effect, simple and quick operation allows the operator to learn to use immediately. The minimum sample volume of 5ml and the residual volume of 1ml can meet more demanding research and development requirements, and can be applied to pharmaceuticals, biotechnology, chemicals, food and nanomaterials Homogeneous applications in industries such as liposomes.